Fig. 2: ZFL induces intracellular Ca2+ release in Caki cells. | Experimental & Molecular Medicine

Fig. 2: ZFL induces intracellular Ca2+ release in Caki cells.

From: Z-FL-COCHO, a cathepsin S inhibitor, enhances oxaliplatin-mediated apoptosis through the induction of endoplasmic reticulum stress

Fig. 2

a, b Caki cell were treated with 2 μM ZFL for the indicated time periods (a) or 2 h (b). After treatment with ZFL, cells were loaded with Flou-4/AM fluorescent dye, and flow cytometry (a) or fluorescence microscopy (b) was used to detect calcium levels. c, d Caki cells were pre-treated with 10 μM BAPTA-AM and 10 μM EGTA-AM for 30 min and were then treated with 2 μM ZFL for 2 h (c) and 8 h (d). Cells were loaded with Fluo-4/AM fluorescent dye, and flow cytometry was used to measurement calcium levels (c). Western blotting was used to detect protein levels of ATF4, CHOP, and actin (d). The values in (a, c) represent the mean ± SD of three independent samples; *p < 0.01 compared to the control; #p < 0.01 compared to ZFL

Back to article page