Fig. 6: Induction of ER stress is associated with ZFL plus oxaliplatin-induced apoptosis. | Experimental & Molecular Medicine

Fig. 6: Induction of ER stress is associated with ZFL plus oxaliplatin-induced apoptosis.

From: Z-FL-COCHO, a cathepsin S inhibitor, enhances oxaliplatin-mediated apoptosis through the induction of endoplasmic reticulum stress

Fig. 6

a Caki cells were pre-treated with 2.5 mM TUDCA and 2.5 mM 4-PBA for 30 min, and then 2 μM ZFL was added for 8 h. b Caki cells were pre-treated with 2.5 mM TUDCA and 2.5 mM 4-PBA for 30 min and then treated with ZFL plus oxaliplatin for 24 h. c Caki cells were transiently transfected with siRNA against the control, ATF4, and CHOP. After 24 h, cells were treated with 2 μM ZFL plus 25 μM oxaliplatin for 24 h. Flow cytometry was used to detect the sub-G1 fraction, and western blotting was used to detect the protein levels of GRP78, ATF4, REDD1, CHOP, PARP, and/or actin. The values in (b, c) represent the mean ± SD of three independent samples; *p < 0.01 compared to the control; #p < 0.01 compared to ZFL plus oxaliplatin; &p < 0.01 compared to ZFL plus oxaliplatin-treated control siRNA

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