Fig. 2: Analysis of PMSC osteogenic and adipogenic differentiation.
From: Notch ligand Jagged1 promotes mesenchymal stromal cell-based cartilage repair
a Osteogenic differentiation was examined by alkaline phosphatase staining and alizarin red staining without (Control) induction or with induction (Induced). Bar indicates 100 µm. b, c Osteogenic differentiation markers Runx2 and osteocalcin were further examined by real-time PCR. d Adipogenic differentiation was studied by the detection of lipid vacuoles by oil red O staining with or without induction. Bar indicates 50 µm. e, f Adipogenic differentiation markers PPARr and C/EBPa were inspected by real-time PCR. The data are the means ± SD of three independent experiments and all the results were normalized to the internal control (*p < 0.05 compared with control PMSCs without induction)
