Fig. 3: The anti-apoptotic effect of melatonin is related to autophagy flux in AGE-treated EPCs. | Experimental & Molecular Medicine

Fig. 3: The anti-apoptotic effect of melatonin is related to autophagy flux in AGE-treated EPCs.

From: Melatonin protects endothelial progenitor cells against AGE-induced apoptosis via autophagy flux stimulation and promotes wound healing in diabetic mice

Fig. 3: The anti-apoptotic effect of melatonin is related to autophagy flux in AGE-treated EPCs.The alternative text for this image may have been generated using AI.

EPCS were untreated or treated with AGEs (400 μg/mL) alone, melatonin (50 μM) + AGEs (400 μg/mL), or AGEs (400 μg/mL) + melatonin (50 μM) combined with chloroquine (CQ; 10 μM). a, b Mitochondrial membrane potential was detected using MitoTracker Red CMXRos, and nuclei were stained with Hoechst. Scale bar, 25 μm. c,e Protein expression levels of LC3, p62, and GAPDH in EPCs of each group treated as described above. f, h Protein expression levels of Bcl-2, Bax, cytochrome c, caspase-9, cleaved caspase-3, and GAPDH in EPCs of each group treated as described above. Data are presented as the mean ± SEM. Significant differences between the treatment and control groups are indicated as **P < 0.01 or *P < 0.05. n = 3

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