Fig. 4: USP15 overexpression promotes the expression of Bcl-2, Bcl-xL, Survivin, and NF-κBp65. | Experimental & Molecular Medicine

Fig. 4: USP15 overexpression promotes the expression of Bcl-2, Bcl-xL, Survivin, and NF-κBp65.

From: USP15 inhibits multiple myeloma cell apoptosis through activating a feedback loop with the transcription factor NF-κBp65

Fig. 4: USP15 overexpression promotes the expression of Bcl-2, Bcl-xL, Survivin, and NF-κBp65.The alternative text for this image may have been generated using AI.

After RPMI 8226 and U266 cells were transfected with siNC plus empty pLVX-Puro (vector), siUSP15#1, siUSP15#2, or pLVX-Puro-USP15, the expression of Bcl-2, Bcl-xL, Survivin, cleaved Caspase-3 (C-Caspase-3), cleaved PARP1 (C-PARP1), nuclear NF-κBp65, and cytoplasmic NF-κBp65 was measured by Real-time PCR (a, b) and/or western blot (c-g). (h) IκB-α and p-IκB-α expression in U266 cells was measured by western blot. IκB-α (i) or NF-κBp65 (j) was immunoprecipitated and immunoblotted in cells with empty pLVX-Puro (vector) or pLVX-Puro-USP15 transfection. ***P < 0.001 compared with vector + siNC

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