Fig. 3: Analysis of osteoclast progenitor cells in the bone marrow, typical marker expression in macrophages, and transmigration of preOCs. | Experimental & Molecular Medicine

Fig. 3: Analysis of osteoclast progenitor cells in the bone marrow, typical marker expression in macrophages, and transmigration of preOCs.

From: Ninjurin1 positively regulates osteoclast development by enhancing the survival of prefusion osteoclasts

Fig. 3: Analysis of osteoclast progenitor cells in the bone marrow, typical marker expression in macrophages, and transmigration of preOCs.

a, b Bone marrow cells were analyzed by FACS. a Representative density plots of Csf1r+ bone marrow cells. b The percent frequency of osteoclast progenitor cells (red box, Csf1r+c-Kit+Cd11bdull) in (A) is shown as the mean ± SD; *P < 0.05. c BMMs were analyzed by FACS. The expression of Csf1r is shown in whole cells (left), and Csf1r+-gated cells were analyzed for Rank ((y-axis) and Cd11b ((x-axis) expression (right). d BMMs transduced with Lifeact-mCherry were cultured with M-CSF and RANKL for 3 days to generate preOCs. Lifeact-mCherry-labeled preOCs were overlaid on a GFP+ MC3T3-E1 osteoblast monolayer with the continued presence of osteoclastogenic cytokines. Z-plane images were acquired after 30 min (upper) or 12 h (lower) via confocal microscopy. Scale bars, 10 μm

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