Fig. 3: IR induces the mitochondrial ROS/Src-dependent invasion pathway.
From: Mitochondrial superoxide dismutase 2 mediates γ-irradiation-induced cancer cell invasion
a H460 cells were transfected with a control or a Bcl-XL-expression vector. After 24 and 48 h of incubation, SOD2 mRNA levels were compared by quantitative real-time PCR (top), and the levels of the indicated proteins were compared by western blotting (bottom). b The indicated cells were irradiated with the specified doses of γ-rays. Western blotting for SOD2 and Bcl-XL was performed at 24 and 48 h after irradiation. c Irradiated (2 Gy, 48 h) and untreated control H460 cells were incubated in the presence or absence of NAC (5 mM) and metformin (5 mM) for 1 h, and mitochondrial ROS levels were compared by staining cells with MitoSOX Red probes and analyzing them by flow cytometry. d Irradiated and untreated control cells were analyzed for their invasiveness in the presence or absence of NAC or metformin. The levels of Src and phosphorylated Src in the cells were compared by western blotting
