Fig. 4: Bcl-XL and SOD2 functionally cooperate to promote Src phosphorylation and cell invasion.
From: Mitochondrial superoxide dismutase 2 mediates γ-irradiation-induced cancer cell invasion
a H460 cells treated with a control, Bcl-XL-targeting, or SOD2-targeting siRNA were irradiated, and the levels of the indicated proteins were compared 24 h after irradiation. b H460 cells infected with lentiviruses expressing the specified shRNAs were transfected with a Bcl-XL or SOD2 expression vector in the indicated combinations. Cellular invasiveness and the levels of Bcl-XL and SOD2 were compared after 48 h of incubation. c H460 cells were transfected with a Bcl-XL or SOD2 expression vector in the indicated combinations. Cellular invasiveness and the levels of the indicated proteins were compared after 48 h of incubation. d H460 cells treated with a control or SOD2-targeting siRNA were irradiated (2 Gy). After 48 h of incubation, irradiated and untreated control cells were analyzed for their levels of O2•− and H2O2 using MitoSox Red and Peroxy Orange-1, respectively. e H460 cells were transfected with a SULF2 expression vector and SOD2-targeting siRNA in the indicated combinations (left). Alternatively, H460 cells infected with lentiviruses expressing a control or SOD2-targeting shRNA were incubated in the presence or absence of 100 ng/mL IL-6 (right). After treatment for 24 h, cellular H2O2 levels were compared using Peroxy Orange-1
