Fig. 1: One-year-old CD4-ERα knockout female mice developed spontaneous mild autoimmune phenotypes. | Experimental & Molecular Medicine

Fig. 1: One-year-old CD4-ERα knockout female mice developed spontaneous mild autoimmune phenotypes.

From: Estrogen receptor α in T cells suppresses follicular helper T cell responses and prevents autoimmunity

Fig. 1: One-year-old CD4-ERα knockout female mice developed spontaneous mild autoimmune phenotypes.

a Naïve CD4 T cells were isolated from the spleen of WT and generated CD4-ERα KO mice, and the ERα protein expression level in CD4 T cells was confirmed by western blotting. b, c The amounts of anti-nuclear antibody in the serum of 1-year-old mice were examined via immunofluorescence of prefixed Hep-2 cells stained with diluted (1:40) sera followed by Alexa Fluor 488-conjugated goat anti-mouse Ig antibody. d–f ELISAs were performed for quantitation of (d) anti-dsDNA, (e) IgM and (f) total IgG in the sera of 1-year-old mice. The values represent the mean ± SEM; n = 5. **P < 0.01

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