Fig. 5: High-affinity antibody production and isotype class switching were increased in female CD4-ERα knockout (KO) mice under an estrogen sufficient environment. | Experimental & Molecular Medicine

Fig. 5: High-affinity antibody production and isotype class switching were increased in female CD4-ERα knockout (KO) mice under an estrogen sufficient environment.

From: Estrogen receptor α in T cells suppresses follicular helper T cell responses and prevents autoimmunity

Fig. 5: High-affinity antibody production and isotype class switching were increased in female CD4-ERα knockout (KO) mice under an estrogen sufficient environment.

a Experimental scheme of NP-OVA immunization and 17β-estradiol (E2) treatment. b Flow cytometric analysis of CD4+CD44+CXCR5+Bcl-6+ follicular helper T (TFH) cells and B220+CD95+GL-7+ germinal center (GC) B cells in inguinal lymph nodes (ILNs) from 8-week-old NP-OVA immunized WT and CD4-ERα KO female mice with 200 µg E2 treatment. c, d ELISAs for NP7-(high affinity) and NP30- (low affinity) specific antibodies were performed to measure (c) IgM, total IgG, (d) IgG1, IgG2b, IgG2c, and IgG3 levels in the sera of NP-OVA-immunized WT and CD4-ERα KO female mice. The values represent the mean ± SEM; n = 4. *P < 0.05; **P < 0.01; and ***P < 0.001

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