Fig. 6 | Experimental & Molecular Medicine

Fig. 6

From: microRNA-328 in exosomes derived from M2 macrophages exerts a promotive effect on the progression of pulmonary fibrosis via FAM13A in a rat model

Fig. 6

M2 macrophage-exosomes carry miR-328 into pulmonary interstitial fibroblasts. a miR-328 expression in macrophages and exosomes examined by RT-qPCR. b miR-328 expression in pulmonary interstitial fibroblasts after a coculture with exosomes, as measured by RT-qPCR, * p < 0.05 vs. pulmonary interstitial fibroblasts cocultured with exosomes. c EdU labeling to examine the proliferation of pulmonary interstitial fibroblasts in the mimic NC-exo and miR-328-exo groups (200 × ). d Quantification of the results in b, *, p < 0.05 vs. the mimic NC-exo group; e Expression of Collagen 1A, Collagen 3A, α-SMA and FAM13A in the mimic NC-exo and miR-328-exo groups measured by RT-qPCR, * p < 0.05 vs. the mimic NC-exo group. miR-328, microRNA-328; NC, negative control; EdU, 5-ethynyl-2′-deoxyuridine; α-SMA, α-smooth muscle actin; RT-qPCR, reverse transcription quantitative polymerase chain reaction; NC, negative control; and exo, exosomes. The results were measurement data and were analyzed using an unpaired t test. The results are expressed as the mean ± standard deviation. The experiment was conducted in triplicate

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