Fig. 4: Effects of PP2A inhibition on LPS-reduced osteoblast differentiation. | Experimental & Molecular Medicine

Fig. 4: Effects of PP2A inhibition on LPS-reduced osteoblast differentiation.

From: Peroxiredoxin II negatively regulates BMP2-induced osteoblast differentiation and bone formation via PP2A Cα-mediated Smad1/5/9 dephosphorylation

Fig. 4

C3H10T1/2 cells were treated with 0.25 μg/ml BMP2, 1 μg/ml LPS, and 3 nM okadaic acid (OA) for 2 days as the template. The expression levels of a Id1 and b Dlx5 were determined using real-time PCR. **p < 0.01 compared with untreated control. #p < 0.05 compared with the BMP2-treated group. *p < 0.05, **p < 0.01 compared with the indicated group. c C3H10T1/2 cells were treated with BMP2, LPS, and OA for 4 days. The upper panel represents the image of staining and the lower panel indicates the relative intensity. **p < 0.01 compared with untreated control. #p < 0.05 compared with BMP2-treated group. *p < 0.05 compared with the indicated group. d Western blot analyses were performed using the indicated antibodies as probes. e Real-time PCR was performed using the total RNA isolated from C3H10T1/2 cells treated with BMP2, LPS, and OA for 2 days as the template. **p < 0.01 compared with the BMP2-treated group. Data are presented as the mean ± SEM of three individual experiments

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