Fig. 7: Inhibition of AQP8 during AP did not affect apoptosis in vitro. | Experimental & Molecular Medicine

Fig. 7: Inhibition of AQP8 during AP did not affect apoptosis in vitro.

From: Inhibition of RIPK1-dependent regulated acinar cell necrosis provides protection against acute pancreatitis via the RIPK1/NF-κB/AQP8 pathway

Fig. 7

a Immunofluorescence staining revealed the expression of Caspase-3, Caspase-8, and Caspase-9 in the cytoplasm of AR42J cells from the Control, AP, AP + si-AQP8 and AP + si-NC groups. The green signal represents Caspase-3, Caspase-8, and Caspase-9 staining. The blue signal represents nuclear DNA staining by DAPI; bar: 200 μm. b A TUNEL assay was used to measure apoptosis in AR42J cells and (d) determine the apoptosis index in the AR42J cells described in Fig. 7b. c The histograms show the IOD of Caspase-3, Caspase-8, and Caspase-9 in the AR42J cells described in Fig. 7a. The data are the means ± S.Ds. (n = 3). *P < 0.05 versus control, ^P < 0.05 versus AP

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