Table 1 Comparison of the effects of 5-FC treatment on the time required to eliminate the CD episomal vectors

From: Efficient exogenous DNA-free reprogramming with suicide gene vectors

Cell line

5-FC (μM)

Minimum number of passages needed for EF-cell generation

Control-iPSC #01a

0

>6

 

50

>6

CD-iPSC #01a

0

>6

 

50

4

CD-iPSC #02b

0

>1

 

50

1

CD-iPSC #03b

0

>2

 

50

2

CD-iPSC #04b

0

>2

 

50

2

CD-iNSCb

0

>3

 

50

1

  1. aThis sample is a mixture of nine independent iPSC colonies
  2. bFor this experiment, we dissociated the colonies into single cells