Table 1 Analysis of personalized cancer-specific rearranged sequences in gastric cancer patients
ID | Sex | Age | Recurrence status | Stage (TNM) | Rearranged sites in WG-NGS | Primers designed for PCR sites | Cancer-specific PCR | Confirmed by Sanger sequencing | Final validated sites | PreOp ctDNA | PostOp ctDNA | Lead time (months) |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
GC1 | M | 68 | R | IIIA (T3N2M0), | 48 | 12 | 7 | 4 | 4 | ā | ā | Ā |
GC4 | M | 67 | R | IIIC (T4aN3bM0) | 44 | 7 | 3 | 3 | 3 | + | + | 9.4 |
GC6 | M | 64 | R | IIIB (T3N3aM0) | 3 | 3 | 3 | 3 | 3 | ā | ā | Ā |
GC7 | M | 57 | R | IIIB (T4aN2M0) | 3 | 3 | 3 | 1 | 1 | ā | Ā | Ā |
GC8 | M | 53 | R | IIIC (T4bN3aM0) | 8 | 6 | 4 | 3 | 2 | + | + | ā1.4 |
GC9 | M | 44 | R | GIST (T4N0M0) | 8 | 8 | 3 | 3 | 3 | + | + | 5.0 |
GC10 | M | 73 | R | IIIB (T4aN2M0) | 31 | 5 | 3 | 3 | 3 | ā | ā | Ā |
GC11 | M | 70 | R | IIIB (T3N3aM0) | 90 | 12 | 6 | 5 | 5 | + | + | 5.6 |
GC12 | M | 71 | R | IIIC (T4aN3aM0) | 3 | 3 | 3 | 2 | 2 | ā | ā | Ā |
GC14 | M | 53 | R | IIIB (T3N3bM0) | 25 | 11 | 4 | 2 | 2 | ā | + | 0.7 |
GC15 | M | 42 | R | IIIC (T4bN3aM0) | 7 | 7 | 5 | 3 | 2 | + | + | 3.1 |
GC17 | M | 71 | R | IV (T4aN3bP1) | 16 | 12 | 8 | 5 | 5 | + | + | a |
GC18 | M | 41 | R | IIB (T3N1M0), | 6 | 5 | 4 | 2 | 2 | ā | ā | Ā |
GC21 | M | 77 | R | IIIC (T4aN3aM0) | 40 | 13 | 6 | 6 | 5 | ā | ā | Ā |
GC22 | M | 49 | R | IV (T4aN3bP1) | 23 | 9 | 3 | 3 | 3 | + | + | a |
GC31 | M | 54 | N | IIA (T3N0M0), | 6 | 5 | 4 | 3 | 3 | + | ā | Ā |
GC32 | M | 70 | R | IIIC (T4bN2M0) | 11 | 8 | 8 | 8 | 8 | + | ā | Ā |
GC33 | M | 44 | N | IIIC (T4bN3bM0) | 6 | 6 | 6 | 6 | 6 | + | ā | Ā |
GC34 | M | 63 | N | IIIC (T4aN3aM0) | 3 | 3 | 1 | 1 | 1 | + | ā | Ā |
CG35b | M | 60 | N | IIA (T3N0M0) | 0 | 0 | 0 | 0 | 0 | Ā | Ā | Ā |
GC2b | F | 62 | R | IIB (T3N1M0) | 0 | 0 | 0 | 0 | 0 | Ā | Ā | Ā |
GC3b | M | 64 | R | IIIB (T4aN2M0) | 0 | 0 | 0 | 0 | 0 | Ā | Ā | Ā |
GC5b | F | 72 | R | IIIC (T4aN3bM0) | 0 | 0 | 0 | 0 | 0 | Ā | Ā | Ā |
GC13b | F | 73 | R | IIIA (T3N2M0) | 0 | 0 | 0 | 0 | 0 | Ā | Ā | Ā |
GC23b | M | 60 | R | IIIA (T3N2M0) | 3 | 3 | 0 | 0 | 0 | Ā | Ā | Ā |
Total | Ā | Ā | Ā | Ā | 384 | 141 | 84 | 66 | 63 | Ā | Ā | Ā |
