Fig. 6: HDAC9 silencing induced cell cycle arrest and apoptosis.
From: Identification of HDAC9 as a viable therapeutic target for the treatment of gastric cancer
a, b Cell cycle analysis of PI-stained mock-, siNC-, and siHDAC9-treated cells for 48 h by flow cytometry. The histogram peaks indicate the G1/G0, S, and G2/M phases of the cell cycle. The results show that HDAC9 silencing induced G1 arrest and reduced the proportion of cells in G2/M phase. c, d HDAC9 inhibition promoted the induction of apoptosis in GC cell lines, as evidenced by the Annexin V-PE/7-AAD assay. The apoptotic index was obtained from the ratio of early to late apoptotic cells. The error bars indicate the means ± SDs; *p < 0.05, **p < 0.01, ***p < 0.001 versus the control group
