Fig. 6: Prevention of tunicamycin (Tm)-induced fat accumulation by pharmacological Sirt6 activation.
From: Deacetylation of XBP1s by sirtuin 6 confers resistance to ER stress-induced hepatic steatosis
a WT and KO mice were treated with fucoidan (5 or 15 mg/kg) three times via oral gavage and injected with Tm (1 mg/kg) for 24 h. b ER stress-associated markers in the livers were examined by western blotting. c, d Hepatic TG accumulation was analyzed using a specific TG assay kit (n = 3–4) or perilipin immunostaining. Bars = 10 μm. e, f HepG2 cells were pretreated with either fucoidan (Fu) or cyanidin at the indicated concentrations prior to Tm (2 μg/ml) treatment for 6 h. Protein levels of total and acetylated XBP1s were determined by western blotting. The values are the mean ± SEM. **p < 0.01 versus vehicle; #p < 0.05 and ##p < 0.01 versus Tm
