Fig. 5: The effects of IL-1β neutralization on ECM remodeling and cell apoptosis in hIAPP-overexpressing NP cells. | Experimental & Molecular Medicine

Fig. 5: The effects of IL-1β neutralization on ECM remodeling and cell apoptosis in hIAPP-overexpressing NP cells.

From: Targeting the IL-1β/IL-1Ra pathways for the aggregation of human islet amyloid polypeptide in an ex vivo organ culture system of the intervertebral disc

Fig. 5

Human NP cells were transfected with pLVX-mCMV-Puro (over-ctr) and pLVX-mCMV-IAPP (over-IAPP) with or without nIL-1β (1 μg/mL) under static compression for one week. a Western blot analysis was used to evaluate the protein expression levels of FAS, FASL, VDAC1, Cyto-c, Bax, Bcl-2, cleaved caspase-3, and caspase-9. GAPDH was used as an internal control. b Western blot analysis was used to evaluate the protein expression levels of MMP3, MMP9, MMP13, ADAMTS5, COL2A1, SOX9, aggrecan, and hIAPP. c–e Representative images of immunofluorescence for caspase-3 c, caspase-9 d, and FAS e. f–h The mRNA expression levels of caspase-3 f, Bax g, and Bcl-2 h were measured by RT-qPCR. β-actin was used as an internal control. i–j Representative images of TUNEL analysis i and the quantitative analysis of the TUNEL-positive cell rate j. The data are presented as the mean ± SD (n = 3). *P < 0.05 vs. the control group. #P < 0.05 vs. the nIL-1β-untreated group. $P < 0.05 vs. the corresponding group

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