Fig. 2: Effects of 3SG and 6SG on VEGF-induced VEGFR-2 phosphorylation.
From: 6′-Sialylgalactose inhibits vascular endothelial growth factor receptor 2-mediated angiogenesis
a, b HUVECs were treated with the indicated doses of 3SG or 6SG for 72 h. The cytotoxicity of 3SG and 6SG in HUVECs was measured by MTT assays, and the results are presented as the mean ± SD. c, e HUVECs were treated with VEGF-A and/or 3SG and 6SG at the indicated doses. pVEGFR-2 was examined by western blot analysis. Total VEGFR-2 was used as a control. d, f Quantitative densitometric analysis of western blots in c and e. The results represent the fold increase versus the positive control (second lane). The graph shows the mean ± SD (n = 3). *P < 0.01 and **P < 0.001 compared with the positive control
