Fig. 3: Effects of 6SG on VEGF-induced signaling and angiogenic features in HUVECs.
From: 6′-Sialylgalactose inhibits vascular endothelial growth factor receptor 2-mediated angiogenesis
a HUVECs were treated with VEGF-A and/or 6SG at the indicated doses. The effect of 6SG on the viability of VEGF-A-treated HUVECs was measured by MTT assays. The graph shows the mean ± SD. *P < 0.001 compared with the negative control (first lane), #P < 0.05 and ##P < 0.001 compared with the positive control (second lane). b HUVECs were treated with VEGF-A and/or 6SG at the indicated doses. The levels of ERK (p-ERK) and Akt (p-Akt) phosphorylation were examined by western blot analysis. Total ERK, total Akt, and GAPDH were used as controls. c Representative images of tube formation by HUVECs treated with VEGF-A (50 ng/ml) and/or 6SG at the indicated doses. d Quantitative analysis of tube length (mm) c. The graph shows the mean ± SD. *P < 0.01 compared with the control (first lane); #P < 0.01 compared with the positive control (second lane). e Representative images of HUVEC migration following treatment with VEGF-A (50 ng/ml) and/or 6SG at the indicated doses. The migrated cells were stained with hematoxylin and eosin. f Quantitative analysis of migrated HUVECs e. The graph shows the mean ± SD. *P < 0.01 compared with the control (first lane); #P < 0.01 compared with the positive control (second lane). g Representative images of actin stress fibers in HUVECs treated with VEGF-A (50 ng/ml) and/or 6SG at the indicated doses. Actin stress fibers were stained with phalloidin (red)
