Fig. 3: Signaling through Il6r regulates HSC development under the normal vasculogenesis.
From: Interleukin-6 signaling regulates hematopoietic stem cell emergence
a Std, Il6r, and Il6 morphants were interrogated by WISH for kdrl, efnb2a, and dlc at 28 hpf. Black arrowheads denote the vascular lines. b Qualitative phenotype distribution of embryos from a scored with medium, high (up), and low (down) expression of these markers. Medium, white bar; high, red bar; low, gray bar. c Maximum projections of the DA region of fli1a:eGFP embryos at 30 hpf injected with Std, Il6r, and Il6 MOs and assayed for immunohistochemistry for GFP (green) and TUNEL (red). Scale bar represents 200 μm. d Embryos from the same experiment as in c were subjected to WISH for runx1 expression at 30 hpf. Black arrowheads denote HSCs in the floor of the DA region. e Qualitative phenotype distribution of embryos from d scored as in b
