Fig. 1: Oxidative stress significantly reduces TXNIP expression and the suppression of RPE cell proliferation.

a ARPE-19 cells were treated with the indicated concentration of H₂O₂ for 4 h. The cells were lysed and subjected to western blotting for the indicated antibodies. b ARPE-19 cells were stimulated with 0.25 mM H₂O₂ for the indicated times. c MTT assay of the viability of ARPE-19 cells after treatment with H₂O₂ compared with that of ARPE-19 cells not treated with H₂O₂. The results shown are representative of three independent experiments. d ARPE-19 cells were treated with the indicated concentration of H₂O₂ for 4 h. The cells were lysed and subjected to western blotting for the indicated antibodies. e ARPE-19 cells were treated with 0.25 mM H₂O₂ for the indicated time periods. Cell proliferation was assessed by the Wst-1 assay. The results shown are representative of three independent experiments. f Immunoblot analysis of cell cycle-related markers in shTXNIP cells. β-Actin was used as an internal loading control.*p < 0.05; **p < 0.01. The error bars indicate the SEM