Fig. 3: Mob1a/b knockout causes a defect in the differentiation of mouse ESCs. | Experimental & Molecular Medicine

Fig. 3: Mob1a/b knockout causes a defect in the differentiation of mouse ESCs.

From: Loss of Mob1a/b impairs the differentiation of mouse embryonic stem cells into the three germ layer lineages

Fig. 3: Mob1a/b knockout causes a defect in the differentiation of mouse ESCs.

a Quantitative PCR for germ layer markers (endoderm: Gata6, Gata4, Sox17; mesoderm: T-brachyury, MixL1, Mesp1, Hand1; ectoderm: Nestin; trophectoderm: Cdx2, Eomes) in mouse ESCs and EBs. b–d Quantitative PCR for germ layer markers at different stages of EB formation. (b, endoderm; c, mesoderm; d, ectoderm). ES, ESCs before EB formation; EB D2, EB after 2-day culture (hanging drop stage); EB D4, EB after 4-day culture (in bacteriological-grade Petri dish); EB D6, EB after 6-day culture (in cell and tissue culture dish). Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001

Back to article page