Fig. 4: The function of Mob1a/b in the differentiation of mouse ESCs is dependent on Yap activity. | Experimental & Molecular Medicine

Fig. 4: The function of Mob1a/b in the differentiation of mouse ESCs is dependent on Yap activity.

From: Loss of Mob1a/b impairs the differentiation of mouse embryonic stem cells into the three germ layer lineages

Fig. 4: The function of Mob1a/b in the differentiation of mouse ESCs is dependent on Yap activity.

a Immunoblot analysis for Yap, Mob1a/b, and Oct4 in lysates from Mob1af/f:Mob1bf/f:CAGGCre-ERTM ESCs after 4-OHT and shYap treatment. GAPDH served as a loading control. b Representative images of EB outgrowth on tissue culture dishes eight days after EB formation. Dotted lines indicate EB outgrowth. Scale bar, 100 μm c, d Quantitative PCR for Mob1a/b, Yap, and pluripotency markers (Oct4, Sox2, and Nanog) on day 6 after EB formation. e Quantitative PCR for germ layer markers (endoderm, Gata6; mesoderm, T-brachyury; ectoderm, Otx2) at the different stages of EB formation. (−) shCon, shControl ESCs without 4-OHT treatment; (+) shCon, 4-OHT-treated shControl ESCs; (−) shYap ESCs without 4-OHT treatment; (+) shYap, 4-OHT-treated shYap ESCs. Data are presented as the mean ± SEM. **P < 0.01, ***P < 0.001

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