Table 1 Primers used to determine the relative expression of corticosteroid signaling pathway genes and housekeeping genes by RT-qPCR and to quantify DNA immunoprecipitation of Gilz by MeDIP.
From: Preterm birth is associated with epigenetic programming of transgenerational hypertension in mice
Name | Forward primer | Reverse primer | |
|---|---|---|---|
Gene expression (RT-qPCR) | 36B4 | AGCGCGTCCCATTGTCTGT | GGGCAGCAGTGGTGGCAGCAGC |
βactin | AAGTACCCCATTGAACATGCGA | CATCTTTTCACGGTTGGCCTTA | |
HPRT1 | AGACTTGCTCGAGATGTCATGAAG | AATCCAGCAGGTCAGCAAAGA | |
18s | CCGTGCCCTTTGTACACACC | CGATCCGAGGGCCTCA | |
Nr3C2 | ATGGAAACCACACGGTGACCT | AGCCTTATCTCCACACACCAAG | |
Nr3C1 | TTCTGTTCATGGCGTGAGTACC | CCCTTGGCACCTATTCCAGTT | |
αENaC | GGAGTCGAAAATCGGCTTCC | TAGAGCAGGCGAGGTGTCG | |
Sgk1 | TCACTTCTCATTCCAGACCGC | ATAGCCCAAGGCACTGGCTA | |
Gilz | CTGCTGTGGAGTTTGTGACATACTAG | CCAGGCAGGCACTTCTAAGCT | |
Methylation DNA immunoprecipitation-qPCR | Gilz Primer 1 | GAAAACTCAGCCCTTGCTATGG | AAAGCCAAGCAAACCAAACAA |
Gilz Primer 2 | CCACTTTCCTGCCCAACAAA | GAAGGAGGGAAGCAAGAAGACA | |
Gilz Primer 3 | CCCTGTGTTTTGCTGGCAATA | ACACTTGAAGCATTGTGTACCACAT | |
Gilz Primer 4 | TTGCACAGGACACAAGAATATATATGAT | GCTCAAAATAGTTGCACGAAACC | |
Gilz Primer 5 | GACTTGTCTAAGTATGGGTTGAATCTACA | GGAGCAAGCTTATACCAGGAAGTT | |
Gilz Primer 6 | CCCATAGTTAGTATGTCATTGATGGAA | CCACGAGGTTGCATTGAATAATAA | |
Gilz Primer 7 | CCCACCATCTCCCTTGGAAT | CCCTCTGCCACCTAGAGCTTT | |
Gilz Primer 8 | CAGATAACACTCCCGACGACCTA | TGCCAACCTCTGGACATTTTAA | |
Gilz Primer 9 | ATTCCTTTTTCTGCCCATGCT | AAAGAAGGCGGCATCTAAGACTT | |
Gilz Primer 10 | GGATGGAGGTTCTCTTTGGATTC | CGTGCTGATAACAGCTCCATCT | |
Gilz Primer 11 | GAGGTAGCTCAGCGGCAAGA | ACTGTACCACATGAGTGCCTTGTT | |
Gilz Primer 12 | AGTTGGCTGGAGAAAGTGAAGAA | GGGCGGTACTGCATTTAAAAGT |
