Fig. 3: Depletion of IDH2 decreases the expression of mitochondrial-related genes and mitochondrial function in the BAT. | Experimental & Molecular Medicine

Fig. 3: Depletion of IDH2 decreases the expression of mitochondrial-related genes and mitochondrial function in the BAT.

From: Isocitrate dehydrogenase 2 protects mice from high-fat diet-induced metabolic stress by limiting oxidative damage to the mitochondria from brown adipose tissue

Fig. 3: Depletion of IDH2 decreases the expression of mitochondrial-related genes and mitochondrial function in the BAT.The alternative text for this image may have been generated using AI.

Four-week-old WT and IDH2KO mice were fed a HFD for 4 weeks (n = 9 per group). a Heat map of the RNA-seq data representing genes downregulated in the IDH2KO group of HFD samples (top) or downregulated in both LFD and HFD (bottom). b Western blot analysis of IDH2 and PGC-1α from the iBAT. GAPDH was used as a loading control. c Expression of mitochondrial biogenesis genes in the iBAT of the WT and IDH2KO groups. d Relative mtDNA content expressed as a function of total genomic DNA (nDNA) in the WT and IDH2KO groups. e Transmission electron microscopy (TEM) showed multiple sphere-shaped mitochondria, which are characteristic of iBAT (n = 5). f Representative time course of the oxygen consumption rates (OCR) of primary brown adipocytes from the WT and IDH2KO mice. *p < 0.05, **p < 0.01, and ***p < 0.001 vs. HFD–WT or LFD–WT. TEM transmission electron microscopy.

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