Fig. 4: Downregulation of lncRNA NEAT1 or overexpression of LATS2 inhibits the progression of FHF in vivo. | Experimental & Molecular Medicine

Fig. 4: Downregulation of lncRNA NEAT1 or overexpression of LATS2 inhibits the progression of FHF in vivo.

From: Long noncoding RNA NEAT1 suppresses hepatocyte proliferation in fulminant hepatic failure through increased recruitment of EZH2 to the LATS2 promoter region and promotion of H3K27me3 methylation

Fig. 4

The protein expression patterns of YAP1 in the nucleus/cytoplasm of rat liver determined by western blot analysis (a, b). Immunohistochemical staining of Ki67 expression in the livers of FHF rats (×400) (c, d). Apoptosis in the livers of FHF rats observed by TUNEL staining (×400) (e, f). The pathological changes in the livers of FHF rats detected by HE staining (×400) (g). *p < 0.05 vs. the FHF+oe-NC group; #p < 0.05 vs. the FHF+sh-NC group. FHF group, FHF rats induced by D-GalN/LPS injection; FHF+oe-NC group, FHF rats treated with oe-NC; FHF+oe-LATS2 group, FHF rats treated with oe-LATS2; FHF+sh-NC group, FHF rats treated with sh-NC; FHF+sh-NEAT1 group, FHF rats treated with sh-NEAT1. Statistical data are described as mean ± standard deviation. One-way analysis of variance was used for comparisons among multiple groups, followed by Tukey’s post hoc test. n = 6 in each group. D-GalN/LPS D-galactosamine/lipopolysaccharide, lncRNA NEAT1 long noncoding RNA nuclear-enriched abundant transcript 1, LATS2 large tumor-suppressor kinase 2, FHF fulminant hepatic failure, TUNEL terminal deoxynucleotidyl transferase (TdT)-mediated 2′-deoxyuridine 5′-triphosphate (dUTP) biotin nick end labeling, HE hematoxylin–eosin.

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