Fig. 2: UVB-induced lipin-1 inhibition is mediated through SREBP-1 downregulation. | Experimental & Molecular Medicine

Fig. 2: UVB-induced lipin-1 inhibition is mediated through SREBP-1 downregulation.

From: UVB-dependent inhibition of lipin-1 protects against proinflammatory responses in human keratinocytes

Fig. 2

NHEKs were treated with different doses of UVB (0, 10, and 20 mJ/cm2) and cultured for 24 h. a Total RNA was extracted from cells, and the relative mRNA levels of SREBP-1 and SREBP-1 target genes (ACACA, ACLY, and SCD1) were measured via qRT-PCR. b Cell lysates were immunoblotted to determine precursor (P) and nuclear (N) forms of SREBP-1. Protein levels of SREBP-1 were normalized to GAPDH. c NHEKs transfected overnight with the vector containing Flag-SREBP-1a, Flag-SREBP-1c cDNA, or empty pcDNA3.1 vector were exposed to 20 mJ/cm2 UVB radiation and cultured for 24 h. The protein levels of lipin-1 were normalized to GAPDH. All data (mean ± SD) represent three independent experiments. Means without a common letter differ; P < 0.05.

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