Fig. 5: UVB-dependent lipin-1 downregulation attenuates UVB-induced IL-6 and IL-8 production. | Experimental & Molecular Medicine

Fig. 5: UVB-dependent lipin-1 downregulation attenuates UVB-induced IL-6 and IL-8 production.

From: UVB-dependent inhibition of lipin-1 protects against proinflammatory responses in human keratinocytes

Fig. 5

NHEKs that were transfected with the vector containing lipin-1 cDNA or pCMV-AC empty vector were exposed to 20 mJ/cm2 UVB and cultured for 24 h. a Culture media were harvested for cytokine array analysis. Representative altered spots were analyzed via densitometry. The data are presented as the mean ± SD values of the intensities of spots in two independent experiments. b Cell lysates were subjected to immunoblot analysis to confirm lipin-1 overexpression. Secreted IL-6 and IL-8 were detected in culture supernatants using specific ELISA kits. c NHEKs that were transfected with lipin-1 or control siRNA for 24 h were exposed to 20 mJ/cm2 UVB and cultured for 24 h. Total RNA was extracted from cells, and relative mRNA levels of IL-6 and IL-8 were measured via qRT-PCR. d Cell lysates were subjected to immunoblot analysis to confirm lipin-1 knockdown. The culture media were harvested, and IL-6 and IL-8 levels were detected via specific ELISA kits. b–d The data (mean ± SD) represent three independent experiments. Means without a common letter differ; P < 0.05.

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