Fig. 7: Propranolol sensitizes UVB-induced IL-6 and IL-8 production. | Experimental & Molecular Medicine

Fig. 7: Propranolol sensitizes UVB-induced IL-6 and IL-8 production.

From: UVB-dependent inhibition of lipin-1 protects against proinflammatory responses in human keratinocytes

Fig. 7

NHEKs that were exposed to 20 mJ/cm2 UVB were treated with different concentrations of propranolol (PNL; 0, 1, 10, and 100 μM) for 24 h. a, b Secreted IL-6 and IL-8 were detected in culture supernatants using specific ELISA kits. All data (mean ± SD) represent three independent experiments. c A reconstituted human skin model was stimulated with 20 mJ/cm2 UVB, treated with 10 μM propranolol, and fixed at 24 h for immunohistochemical analyses. IL-6 and IL-8 levels were determined using each antibody. Scale bars: 50 μm. d Culture media were analyzed by ELISA kits for IL-6 or IL-8. All data (mean ± SD) represent three independent experiments. Means without a common letter differ; P < 0.05.

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