Fig. 3: The effects of the PLCE1 knockdown on Rho GTPase activity.
From: PLCE1 regulates the migration, proliferation, and differentiation of podocytes
a Active GTP-bound Rac1 and Cdc42 precipitated from podocytes transfected with PLCE1 siRNA using a GST-PAK1 (CRIB domain) pulldown assay. Ponceau red staining at the top shows the GST proteins used. Compared with control podocytes, podocytes transfected with PLCE1 siRNA exhibited a significant decrease in GTP-bound Rac1 and Cdc42 levels. b, c Quantification of Rac1 (b) and Cdc42 (c) in PLCE1 knockdown cells compared to that in control cells. d Active GTP-bound RhoA precipitated from podocytes transfected with PLCE1 siRNA using a GST-rhotekin (RBD domain) pulldown assay. Cells transfected with or without PLCE1 siRNA exhibited no significant differences in GTP-bound RhoA. e Quantification of RhoA in PLCE1 knockdown cells compared with that in control cells. Error bars indicate the standard deviations for more than three independent experiments. *P < 0.05; n.s. not significant.
