Fig. 1: RCAN1.4 was upregulated in human degenerative NP samples. | Experimental & Molecular Medicine

Fig. 1: RCAN1.4 was upregulated in human degenerative NP samples.

From: The RCAN1.4-calcineurin/NFAT signaling pathway is essential for hypoxic adaption of intervertebral discs

Fig. 1: RCAN1.4 was upregulated in human degenerative NP samples.

a Hematoxylin & eosin, safranin O/fast green, and Alcian blue staining of normal and degenerative human lumbar NP tissues is shown. b Gene expression of RCAN1.1, RCAN1.4 SOX9, type II collagen (Col2a1), aggrecan (ACAN), MMP13, and MMP3 in the Pfirrmann grade I/II, III, IV, and V groups (n = 8). c Cells immunopositive for RCAN1 in the human lumbar normal and degenerative NP group are shown. The quantified results are shown on the right (n = 8). d The protein level of RCAN1.4 was higher than that of RCAN1.1 and progressively increased from the control group to the grade V group. e The upregulated RCAN1 and downregulated HIF-1α expression in a tail-looping disc degeneration rat model. Results of quantitative analysis of the percentage of positive cells among the NP cells are shown on the right (n = 4). All experiments were performed at least three times. Scale bar: 100 μm. *P < 0.05, **P < 0.01, ***P < 0.005.

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