Fig. 4: MiR-320a downregulated osteogenic differentiation and inhibited the β-catenin signaling pathway. | Experimental & Molecular Medicine

Fig. 4: MiR-320a downregulated osteogenic differentiation and inhibited the β-catenin signaling pathway.

From: LncRNA DANCR and miR-320a suppressed osteogenic differentiation in osteoporosis by directly inhibiting the Wnt/β-catenin signaling pathway

Fig. 4

Osteogenic differentiation was evaluated at days 7 and 14 by staining the cells with a ALP or d Alizarin Red. The cells were observed under an inverted microscope. b The staining results were further confirmed by ALP activity quantification. c qRT-PCR was performed to evaluate the mRNA expression of ALP. e Western blotting was performed to evaluate the expression of β-catenin in the cytoplasm and nucleus, TCF-1, RUNX2, OPN, and OCN. f Relative mRNA levels of TCF-1, RUNX2, OPN, and OCN upon lentiviral infection were determined by qRT-PCR. BMSCs were infected with lentivirus encoding miR-320a inhibitor or mimics or with empty viruses, and cells were collected and lysed at day 7 and day 14. All experiments were conducted independently at least three times. *P < 0.05 and **P < 0.01.

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