Fig. 6: DANCR overexpression attenuated the osteogenic differentiation induced by the miR-320a inhibitor. | Experimental & Molecular Medicine

Fig. 6: DANCR overexpression attenuated the osteogenic differentiation induced by the miR-320a inhibitor.

From: LncRNA DANCR and miR-320a suppressed osteogenic differentiation in osteoporosis by directly inhibiting the Wnt/β-catenin signaling pathway

Fig. 6

BMSCs were infected with lentivirus encoding miR-320a inhibitor and/or DANCR overexpression vector, followed by incubation in osteogenic differentiation induction medium for 7 or 14 days. Cells were subsequently stained with a ALP and d Alizarin Red and observed under an inverted microscope. b Cells were lysed, and the relative ALP activity was detected. c Relative mRNA expression of ALP was determined by qRT-PCR. All experiments were conducted independently at least three times. *P < 0.05 and **P < 0.01.

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