Fig. 6: TNF-α inhibits EPC differentiation via NF-κB-dependent biogenesis of miR-31/155. | Experimental & Molecular Medicine

Fig. 6: TNF-α inhibits EPC differentiation via NF-κB-dependent biogenesis of miR-31/155.

From: NF-κB-dependent miR-31/155 biogenesis is essential for TNF-α-induced impairment of endothelial progenitor cell function

Fig. 6

CBMNCs (MNCs) were transfected with negative control (NC), NF-κB p65 siRNA (sip65), or miR-31/155 mimics (31 m/155 m), followed by treatment with or without TNF-α. a Representative images of EPCs characterized by double-positive staining with Dil-ac-LDL and FITC-UEA-1. b Cells were labeled with antibodies against EPC marker proteins and analyzed by flow cytometry (n = 3). c, d Tube formation of EPCs in response to VEGF-A was photographed using an inverted microscope and quantitated using ImageJ software (n = 4). e, f Matrigel containing low-dose VEGF-A (5 ng/ml) was mixed with or without 2 × 105 EPCs differentiated from CBMNCs and subcutaneously injected into athymic nude mice. e After 8 days, the Matrigel plugs were harvested and photographed. f The hemoglobin (Hb) content in Matrigel plugs was determined using Drabkin’s reagent (n = 4). *P < 0.05, **P < 0.01, and ***P < 0.001.

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