Fig. 7: miR-31/155 mimics and an eNOS inhibitor impair EPC differentiation. | Experimental & Molecular Medicine

Fig. 7: miR-31/155 mimics and an eNOS inhibitor impair EPC differentiation.

From: NF-κB-dependent miR-31/155 biogenesis is essential for TNF-α-induced impairment of endothelial progenitor cell function

Fig. 7

a–e CBMNCs were transfected with negative control (NC), miR-31 mimic (miR-31m or 31 m), or miR-155 mimic (miR-155m or 155 m) and differentiated into EPCs in fresh media. a The levels of eNOS protein and NO production were determined by western blotting and confocal microscopy (n = 4). b Representative images of EPCs characterized by double-positive staining with Dil-ac-LDL and FITC-UEA-1. c The expression levels of EPC markers were analyzed by flow cytometry (n = 3). d, e The angiogenic potential of EPCs was determined by a tube formation assay (n = 4). f–h Adherent CBMNCs were cultured with or without L-NAME (1 mM) for 4 days. f Intracellular NO production was determined by confocal microscopy (n = 4). g EPC differentiation was confirmed by staining with Dil-ac-LDL and FITC-UEA-1. h The expression levels of EPC markers were analyzed by flow cytometry (n = 3). *P < 0.05, **P < 0.01, and ***P < 0.001.

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