Fig. 1: Development of the central nervous system in zebrafish.

a Detection of early neuronal precursor cells by whole-mount in situ hybridization with a pan-neuronal marker, huC, at the neural plate stage (10.5 h after fertilization). Unpublished data. b Immunostaining of axonal growth in the spinal cord of one-day-old zebrafish. Double-staining with anti-gicerin antibody and anti-HNK-1 antibody28. c Confocal image of myelin structure in an isolated adult zebrafish brain visualized by mbp promoter-driven membrane-tagged GFP, Tg(mbp:mEGFP). Arrows indicate the olfactory, optic, and otic nerves. Unpublished data.