Fig. 5: The distribution of endogenous mGlu7 is altered by MLN4924 treatment.
From: Neddylation is required for presynaptic clustering of mGlu7 and maturation of presynaptic terminals
Primary hippocampal neurons (DIV14) were treated with DMSO or 1 μM MLN4924 for 6 or 12 h. The neurons were fixed and coimmunostained with anti-mGlu7 and anti-bassoon antibodies. Representative images of neurites are presented. Scale bar, 5 μm. The summary quantification graph with scatter plots shows the mean ± SEM (mGlu7 puncta, DMSO: 6.33 ± 0.34, 6 h: 5.91 ± 0.29, 12 h: 6.02 ± 0.43; bassoon puncta, DMSO: 7.47 ± 0.37, 6 h: 7.31 ± 0.32, 12 h: 7.47 ± 0.45; mGlu7 puncta colocalized with bassoon, DMSO: 3.42 ± 0.33, 6 h: 2.20 ± 0.23, 12 h: 1.73 ± 0.25; n = 3, total dendrites = 15; *p < 0.05, n.s. indicates p > 0.05 versus DMSO, one-way ANOVA followed by Tukey’s post hoc test).
