Fig. 1: ZIP8 is upregulated in the inflamed joint tissues of CIA mice and RA patients.
From: ZIP8 exacerbates collagen-induced arthritis by increasing pathogenic T cell responses
a, b Zinc levels in the serum (a) and knee joint synovial fluid (b) of CIA mice and nonimmunized (NI) control mice (N = 10 mice per group). c Representative images of cellular zinc levels in the joint tissues of CIA and NI mice (N = 5 mice per group). c, cartilage; s, synovium. d Relative mRNA levels of ZIP family (zinc importer) and ZNT family (zinc exporter) members in total synovial cells were quantified by qRT-PCR analysis (N = 6 mice per group). The error bars represent ±SD, and unpaired two-tailed Student’s t-tests were performed to determine differences between groups (a–d). e Representative images of immunostaining for MMP3, ZIP8, MTF1, and MT1/MT2 in the synovium of CIA and NI mice (N = 5 mice per group). f Microarray analysis of the indicated ZIP family members in synovial tissues from patients with rheumatoid arthritis (RA; N = 33), patients with osteoarthritis (OA; N = 26), and healthy individuals (HE; N = 20). Microarray data were obtained from the Gene Expression Omnibus database at the National Center for Biotechnology Information (NCBI). The box indicates the 25th and 75th percentiles, with the centerline representing the mean; significance was analyzed by one-way ANOVA followed by Bonferroni’s post hoc comparison. *P < 0.05; **P < 0.005; ***P < 0.0005; ns, not significant. Scale bars: 50 μm.
