Fig. 2: ZIP8 regulates CIA in mice.
From: ZIP8 exacerbates collagen-induced arthritis by increasing pathogenic T cell responses
a Cumulative CIA incidence (score 1) on the indicated days after the first immunization of WT, Slc39a8+/−, Mtf1+/−, and Mt1−/−Mt2−/− mice. Mean ± SEM are shown. b Severity of CIA in the indicated CIA mice, as assessed by measuring the clinical score. c Type II collagen-specific autoantibody production under NI and CIA conditions in the sera of the indicated mice. d Scoring of synovial inflammation (synovitis) in the indicated CIA mice. e Representative images of Ki67 staining to detect proliferating cells (N = 8 mice per group). Scale bars: 50 μm. f Number of blood vessels in the synovium of the indicated mice under NI and CIA conditions. The numbers of mice used were as follows: 20 WT, Slc39a8+/−, and Mtf1+/− mice and 10 Mt1−/−Mt2−/− mice (a, b, d). Eight mice per group were used in the experiments presented in (c, e, and f). All parameters in (b–f) were determined on day 36 after the first immunization. The values are presented as mean ± 95% CI and were assessed with the Mann-Whitney U test (b, d); the values are presented as mean ± SEM. and were assessed with ANOVA and Bonferroni’s post hoc comparison (d, f). *P < 0.05, **P < 0.005, ***P < 0.0005. ns: not significant.
