Fig. 3: Among the infiltrated immune cells, ZIP8 is predominantly expressed in CD4+ T cells.
From: ZIP8 exacerbates collagen-induced arthritis by increasing pathogenic T cell responses
a, b Typical immunofluorescence microscopic images of DAPI, ZIP8, and markers for B cells (B220), T cells (CD4), macrophages (CD11b), and fibroblast-like synoviocytes (FLSs, vimentin) in synovial tissues (left) and primary cultures of total synovial cells isolated from CIA mice (right) (a). The percentage of ZIP8-positive cells was determined by immunofluorescence microscopic analysis of primary cultures of total synovial cells isolated from CIA mice (b N = 5 mice). c Flow cytometric analysis of ZIP8 expression on CD4+ cells, CD11b+ cells, and B220+ cells from total synovial cells isolated from the knee joints of CIA mice. The data shown in (a, c) are representative of five independent experiments.
