Table 2 Gut microbiome enzymes involved in mucin degradation134,135.

From: Slimy partners: the mucus barrier and gut microbiome in ulcerative colitis

Major phylum

Organism

Domains

PDB entry

Mucolytic enzyme

Bacteroidetes

Alistipes finegoldii DSM 17242

GH2, GH20,GH29

 

β-galactosidase (EC 3.2.1.23); β-1,6-N-acetylglucosaminidase (EC 3.2.1.-), β-6-SO3-N-acetylglucosaminidase (EC 3.2.1.-);α-1,3/1,4-L-fucosidase (EC 3.2.1.111)

Bacteroides caccae ATCC 43185

GH2, GH20, GH29, GH33, GH35, GH84, GH89, GH95

 

β-galactosidase (EC 3.2.1.23); β-1,6-N-acetylglucosaminidase (EC 3.2.1.-); β-6-SO3-N-acetylglucosaminidase (EC 3.2.1.-); α-1,3/1,4-L-fucosidase (EC 3.2.1.111); sialidase or neuraminidase (EC 3.2.1.18); β-1,3-galactosidase (EC 3.2.1.-); [protein]-3-O-(GlcNAc)-L-Ser/Thr β-N-acetylglucosaminidase (EC 3.2.1.169); α-N-acetylglucosaminidase (EC 3.2.1.50); α-1,2-L-fucosidase (EC 3.2.1.63)

Bacteroides thetaiotaomicron VPI-5482

GH2, GH20, GH29, GH33, GH35, GH42, GH84, GH89, GH95,

4BBW (GH33); GH29 (3EYP, 4OUE, 4OZO); GH84(2CHN, 2CHO, 2J47, 2J4G, 2JIW, 2 VVN, 2 VVS, 2 W4X, 2 W66, 2 W67, 2 WCA, 2 WZH, 2 WZI, 2X0H, 2XJ7, 2XM1, 2XM2, 4AIS,4AIU)

β-galactosidase (EC 3.2.1.23); β-1,6-N-acetylglucosaminidase (EC 3.2.1.-); β-6-SO3-N-acetylglucosaminidase (EC 3.2.1.-); α-1,3/1,4-L-fucosidase (EC 3.2.1.111); sialidase or neuraminidase (EC 3.2.1.18); β-1,3-galactosidase (EC 3.2.1.-); β-galactosidase (EC 3.2.1.23); [protein]-3-O-(GlcNAc)-L-Ser/Thr β-N-acetylglucosaminidase (EC 3.2.1.169); α-N-acetylglucosaminidase (EC 3.2.1.50); α-1,2-L-fucosidase (EC 3.2.1.63)

Bacteroides xylanisolvens H207

GH2, GH20, GH29, GH33, GH35, GH42, GH89, GH95

—

β-galactosidase (EC 3.2.1.23); β-1,6-N-acetylglucosaminidase (EC 3.2.1.-); β-6-SO3-N-acetylglucosaminidase (EC 3.2.1.-); α-1,3/1,4-L-fucosidase (EC 3.2.1.111); sialidase or neuraminidase (EC 3.2.1.18); β-1,3-galactosidase (EC 3.2.1.-); β-galactosidase (EC 3.2.1.23); α-N-acetylglucosaminidase (EC 3.2.1.50); α-1,2-L-fucosidase (EC 3.2.1.63)

Odoribacter splanchnicus DSM 20712

GH2, GH20, GH29, GH95,

—

β-galactosidase (EC 3.2.1.23); β-1,6-N-acetylglucosaminidase (EC 3.2.1.-); β-6-SO3-N-acetylglucosaminidase (EC 3.2.1.-); α-1,3/1,4-L-fucosidase (EC 3.2.1.111); α-1,2-L-fucosidase (EC 3.2.1.63)

Parabacteroides distasonis ATCC 8503

GH2, GH20, GH29, GH33, GH95

—

β-galactosidase (EC 3.2.1.23); β-1,6-N-acetylglucosaminidase (EC 3.2.1.-); β-6-SO3-N-acetylglucosaminidase (EC 3.2.1.-); β-1,3-galactosidase (EC 3.2.1.-); α-1,2-L-fucosidase (EC 3.2.1.63)

Prevotella denticola F0289

GH2, GH20, GH29, GH33, GH84, GH85, GH95

—

β-galactosidase (EC 3.2.1.23); β-1,6-N-acetylglucosaminidase (EC 3.2.1.-); β-6-SO3-N-acetylglucosaminidase (EC 3.2.1.-); α-1,3/1,4-L-fucosidase (EC 3.2.1.111); sialidase or neuraminidase (EC 3.2.1.18); [protein]-3-O-(GlcNAc)-L-Ser/Thr β-N-acetylglucosaminidase (EC 3.2.1.169); endo-β-N-acetylglucosaminidase (EC 3.2.1.96); α-1,2-L-fucosidase (EC 3.2.1.63)

Bacteroides fragilis 638 R

GH2, GH20,GH29, GH33, GH35, GH84, GH89, GH95

—

β-galactosidase (EC 3.2.1.23); β-1,6-N-acetylglucosaminidase (EC 3.2.1.-); β-6-SO3-N-acetylglucosaminidase (EC 3.2.1.-); α-1,3/1,4-L-fucosidase (EC 3.2.1.111); sialidase or neuraminidase (EC 3.2.1.18); β-1,3-galactosidase (EC 3.2.1.-); [protein]-3-O-(GlcNAc)-L-Ser/Thr β-N-acetylglucosaminidase (EC 3.2.1.169); α-N-acetylglucosaminidase (EC 3.2.1.50); α-1,2-L-fucosidase (EC 3.2.1.63)

Firmicutes

Ruminococcus bromii L2-63

—

—

 

Ruminococcus torques

GH2, GH95

—

β-galactosidase (EC 3.2.1.23); α-1,2-L-fucosidase (EC 3.2.1.63);

Ruminococcus sp. SR1/5

GH2, GH29, GH42

—

β-galactosidase (EC 3.2.1.23); α-1,3/1,4-L-fucosidase (EC 3.2.1.111); β-galactosidase (EC 3.2.1.23)

Ruminococcus bicirculans 80/3

GH2, GH95

—

β-galactosidase (EC 3.2.1.23); α-1,2-L-fucosidase (EC 3.2.1.63)

Streptococcus thermophilus

GH2

—

β-galactosidase (EC 3.2.1.23)

Streptococcus sanguinis CGMH010

—

—

 

Streptococcus oralis

—

—

sulfatase

Clostridium perfringens ATCC 13124

GH2, GH20, GH29, GH33, GH84, GH85, GH89, GH95, GH101

4 L2E(GH33)

β-galactosidase (EC 3.2.1.23); β-1,6-N-acetylglucosaminidase (EC 3.2.1.-); β-6-SO3-N-acetylglucosaminidase (EC 3.2.1.-); α-1,3/1,4-L-fucosidase (EC 3.2.1.111); sialidase or neuraminidase (EC 3.2.1.18); [protein]-3-O-(GlcNAc)-L-Ser/Thr β-N-acetylglucosaminidase (EC 3.2.1.169); endo-β-N-acetylglucosaminidase (EC 3.2.1.96); α-N-acetylglucosaminidase (EC 3.2.1.50); α-1,2-L-fucosidase (EC 3.2.1.63); endo-α-N-acetylgalactosaminidase (EC 3.2.1.97)

Lactobacillus reuteri 1B

GH2

—

β-galactosidase (EC 3.2.1.23)

Lactobacillus plantarum 10CH

GH2, GH20, GH42

—

β-galactosidase (EC 3.2.1.23); β-1,6-N-acetylglucosaminidase (EC 3.2.1.-); β-6-SO3-N-acetylglucosaminidase (EC 3.2.1.-); β-galactosidase (EC 3.2.1.23);

Lactobacillus rhamnosus 4B15

GH2, GH29, GH35

 

β-galactosidase (EC 3.2.1.23); α-1,3/1,4-L-fucosidase (EC 3.2.1.111); β-1,3-galactosidase (EC 3.2.1.-)

Blautia hansenii DSM 20583

GH2, GH20, GH29, GH33, GH84, GH85, GH95, GH101

—

β-galactosidase (EC 3.2.1.23); β-1,6-N-acetylglucosaminidase (EC 3.2.1.-); β-6-SO3-N-acetylglucosaminidase (EC 3.2.1.-); α-1,3/1,4-L-fucosidase (EC 3.2.1.111); sialidase or neuraminidase (EC 3.2.1.18); [protein]-3-O-(GlcNAc)-L-Ser/Thr β-N-acetylglucosaminidase (EC 3.2.1.169); endo-β-N-acetylglucosaminidase (EC 3.2.1.96); α-1,2-L-fucosidase (EC 3.2.1.63); α-1,2-L-fucosidase (EC 3.2.1.63); endo-α-N-acetylgalactosaminidase (EC 3.2.1.97)

Butyrivibrio fibrisolvens 16/4

GH2, GH35, GH42

—

β-galactosidase (EC 3.2.1.23); β-1,3-galactosidase (EC 3.2.1.-); β-galactosidase (EC 3.2.1.23)

Eubacterium rectale ATCC 33656

GH2, GH42

—

β-galactosidase (EC 3.2.1.23); β-galactosidase (EC 3.2.1.23)

Eubacterium siraeum 70/3

GH2, GH95

—

β-galactosidase (EC 3.2.1.23); α-1,2-L-fucosidase (EC 3.2.1.63)

Faecalibacterium prausnitzii 942/30-2

GH2

—

β-galactosidase (EC 3.2.1.23)

Roseburia intestinalis L1-82

GH2, GH20, GH29, GH35, GH42, GH85, GH95

—

β-galactosidase (EC 3.2.1.23); β-1,6-N-acetylglucosaminidase (EC 3.2.1.-); β-6-SO3-N-acetylglucosaminidase (EC 3.2.1.-); α-1,3/1,4-L-fucosidase (EC 3.2.1.111); β-1,3-galactosidase (EC 3.2.1.-); β-galactosidase (EC 3.2.1.23); endo-β-N-acetylglucosaminidase (EC 3.2.1.96); α-1,2-L-fucosidase (EC 3.2.1.63)

Proteobacteria

Burkholderia ambifaria AMMD

GH2, GH20, GH42

—

β-galactosidase (EC 3.2.1.23); β-1,6-N-acetylglucosaminidase (EC 3.2.1.-); β-6-SO3-N-acetylglucosaminidase (EC 3.2.1.-); β-galactosidase (EC 3.2.1.23)

Pseudomonas aeruginosa 12-4-4(59)

—

—

sulfatase

Shigella flexneri 113

GH2

—

β-galactosidase (EC 3.2.1.23)

Vibrio cholerae 569B 395

1 W0

1W0P, 1 W0O (GH33)

sialidase or neuraminidase (EC 3.2.1.18)

Vibrio cholerae 10432-62

GH2, GH20, GH33

—

β-galactosidase (EC 3.2.1.23); β-1,6-N-acetylglucosaminidase (EC 3.2.1.-); β-6-SO3-N-acetylglucosaminidase (EC 3.2.1.-); sialidase or neuraminidase (EC 3.2.1.18)

Proteus vulgaris biosolid 26

GH33

—

sialidase or neuraminidase (EC 3.2.1.18)

Klebsiella oxytoca AR_0028

GH2, GH42

—

β-galactosidase (EC 3.2.1.23); β-galactosidase (EC 3.2.1.23)

Enterobacter cloacae 109

GH2, GH20

—

β-galactosidase (EC 3.2.1.23); β-1,6-N-acetylglucosaminidase (EC 3.2.1.-); β-6-SO3-N-acetylglucosaminidase (EC 3.2.1.-)

Desulfovibrio desulfuricans ATCC 27774

—

—

 

Escherichia coli HS

GH2

—

β-galactosidase (EC 3.2.1.23)

Escherichia coli

GH2

—

β-galactosidase (EC 3.2.1.23)

Actinobacteria

Bifidobacterium angulatum DSM 20098 = JCM 7096

GH2, GH42

—

β-galactosidase (EC 3.2.1.23); β-galactosidase (EC 3.2.1.23)

Bifidobacterium longum subsp. infantis ATCC 15697

GH2, GH20, GH29, GH35, GH42, GH95

3 MO4 (GH29)

β-galactosidase (EC 3.2.1.23); -1,6-N-acetylglucosaminidase (EC 3.2.1.-); β-6-SO3-N-acetylglucosaminidase (EC 3.2.1.-); α-1,3/1,4-L-fucosidase (EC 3.2.1.111); β-1,3-galactosidase (EC 3.2.1.-); β-galactosidase (EC 3.2.1.23); α-1,2-L-fucosidase (EC 3.2.1.63)

Bifidobacterium longum subsp. longum JDM301

GH2, GH20, GH29, GH35, GH42, GH85, GH95

—

β-galactosidase (EC 3.2.1.23); -1,6-N-acetylglucosaminidase (EC 3.2.1.-); β-6-SO3-N-acetylglucosaminidase (EC 3.2.1.-); α-1,3/1,4-L-fucosidase (EC 3.2.1.111); β-1,3-galactosidase (EC 3.2.1.-); β-galactosidase (EC 3.2.1.23); endo-β-N-acetylglucosaminidase (EC 3.2.1.96); α-1,2-L-fucosidase (EC 3.2.1.63)

Bifidobacterium bifidum JCM 1254

GH2, GH20, GH29, GH33, GH42, GH89, GH95

2EAB, 2EAC, 2EAD, 2EAE(GH95)

β-galactosidase (EC 3.2.1.23); β-1,6-N-acetylglucosaminidase (EC 3.2.1.-); β-6-SO3-N-acetylglucosaminidase (EC 3.2.1.-); α-1,3/1,4-L-fucosidase (EC 3.2.1.111); sialidase or neuraminidase (EC 3.2.1.18); β-galactosidase (EC 3.2.1.23); α-N-acetylglucosaminidase (EC 3.2.1.50); α-N-acetylgalactosaminidase (EC 3.2.1.49)

Bifidobacterium adolescents ATCC 15703

GH2, GH35, GH42

—

β-galactosidase (EC 3.2.1.23); β-1,3-galactosidase (EC 3.2.1.-); β-galactosidase (EC 3.2.1.23)

Bifidobacterium catenulatum DSM 16992 = JCM 1194 = LMG 11043

GH2, GH42

—

β-galactosidase (EC 3.2.1.23); β-galactosidase (EC 3.2.1.23)

Bifidobacterium breve UCC2003

GH2, GH20, GH33, GH35, GH42, GH95, GH129

—

β-galactosidase (EC 3.2.1.23); β-1,6-N-acetylglucosaminidase (EC 3.2.1.-); β-6-SO3-N-acetylglucosaminidase (EC 3.2.1.-); sialidase or neuraminidase (EC 3.2.1.18); β-1,3-galactosidase (EC 3.2.1.-); β-galactosidase (EC 3.2.1.23); α-1,2-L-fucosidase (EC 3.2.1.63); α-N-acetylgalactosaminidase (EC 3.2.1.49)

Rothia mucilaginosa DY-18

—

—

—

Bifidobacterium animalis BL3

GH2, GH42

 

β-galactosidase (EC 3.2.1.23); β-galactosidase (EC 3.2.1.23)

Verrucomicrobia

Akkermansia muciniphila ATCC BAA-835

GH2, GH20, GH29, GH33, GH35, GH84, GH89, GH95

 

β-galactosidase (EC 3.2.1.23); β-1,6-N-acetylglucosaminidase (EC 3.2.1.-); β-6-SO3-N-acetylglucosaminidase (EC 3.2.1.-); α-1,3/1,4-L-fucosidase (EC 3.2.1.111); sialidase or neuraminidase (EC 3.2.1.18); β-1,3-galactosidase (EC 3.2.1.-); [protein]-3-O-(GlcNAc)-L-Ser/Thr β-N-acetylglucosaminidase (EC 3.2.1.169);α-N-acetylglucosaminidase (EC 3.2.1.50); -1,2-L-fucosidase (EC 3.2.1.63)

Akkermansia muciniphila YL44

GH2, GH20, GH29, GH33, GH35, GH84, GH89, GH95

—

β-galactosidase (EC 3.2.1.23); β-1,6-N-acetylglucosaminidase (EC 3.2.1.-); β-6-SO3-N-acetylglucosaminidase (EC 3.2.1.-); α-1,3/1,4-L-fucosidase (EC 3.2.1.111); sialidase or neuraminidase (EC 3.2.1.18); β-1,3-galactosidase (EC 3.2.1.-); [protein]-3-O-(GlcNAc)-L-Ser/Thr β-N-acetylglucosaminidase (EC 3.2.1.169);α-N-acetylglucosaminidase (EC 3.2.1.50); -1,2-L-fucosidase (EC 3.2.1.63)

Akkermansia glycaniphila

GH2, GH20, GH29, GH33, GH35, GH84, GH89, GH95

—

β-galactosidase (EC 3.2.1.23); β-1,6-N-acetylglucosaminidase (EC 3.2.1.-); β-6-SO3-N-acetylglucosaminidase (EC 3.2.1.-); α-1,3/1,4-L-fucosidase (EC 3.2.1.111); sialidase or neuraminidase (EC 3.2.1.18); β-1,3-galactosidase (EC 3.2.1.-); [protein]-3-O-(GlcNAc)-L-Ser/Thr β-N-acetylglucosaminidase (EC 3.2.1.169); α-N-acetylglucosaminidase (EC 3.2.1.50); α-1,2-L-fucosidase (EC 3.2.1.63)

Akkermansia muciniphila AMDK-3

GH2, GH20, GH29, GH33, GH35, GH84, GH89, GH95

—

β-galactosidase (EC 3.2.1.23); β-1,6-N-acetylglucosaminidase (EC 3.2.1.-); β-6-SO3-N-acetylglucosaminidase (EC 3.2.1.-); α-1,3/1,4-L-fucosidase (EC 3.2.1.111); sialidase or neuraminidase (EC 3.2.1.18); β-1,3-galactosidase (EC 3.2.1.-); [protein]-3-O-(GlcNAc)-L-Ser/Thr β-N-acetylglucosaminidase (EC 3.2.1.169); α-N-acetylglucosaminidase (EC 3.2.1.50); α-1,2-L-fucosidase (EC 3.2.1.63)

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