Fig. 1: The inflammatory effect of OEA on LTE4-pretreated A549 cells.
From: Oleoylethanolamide induces eosinophilic airway inflammation in bronchial asthma
The cells were treated with LTE4 (100 nM) and OEA (100 μM) for 24 h or were pretreated with LTE4 (100 nM) for 6 h and subsequently treated with OEA (100 μM) for 18 h. a The mRNA expression (IL-1β, IL-6, IL-8, IL-25, and IL-33) and b production of inflammatory cytokines (IL-6, IL-8, and IL-33) was measured by quantitative real-time PCR and ELISA. The data are presented as fold changes compared with the control group (set at 1) and as the means ± SD of three separate experiments performed in duplicate (n = 4–6). P values were analyzed by the Mann–Whitney test. ***P < 0.001, **P < 0.01, *P < 0.05 vs. NC; ##P < 0.01, #P < 0.05 vs. OEA; @@@P < 0.001, @@P < 0.01, @P < 0.05 vs. LTE4.
