Fig. 2: RBM24 promotes the proliferation of BC cells in vitro.
From: RBM24 exacerbates bladder cancer progression by forming a Runx1t1/TCF4/miR-625-5p feedback loop
a Western blot analysis was used to examine the protein level of RBM24 in human uroepithelial cells (SV-HUC-1) and BC cell lines (UM-UC-3, 253 J, T24, and J82). Densitometric analysis (A) was performed in three independent experiments. *P < 0.05, **P < 0.01 vs. the SV-HUC-1 group. b RT-qPCR was used to detect the mRNA expression of RBM24 in the cell lines. *P < 0.05 vs. the SV-HUC-1 group. c The RBM24 and CDK4 protein levels were measured by western blot analysis in shRBM24-transfected 253 J cells or RBM24 overexpression vector (oeRBM24)-transfected J82 cells. *P < 0.05, **P < 0.01 vs. the corresponding control. d Cells were prepared as in (c), and cell viability was measured by MTT assay. *P < 0.05 vs the corresponding control. e Cells were prepared as in (c), and cell viability was measured by colony formation assays. The right panel shows the numbers of colonies formed. *P < 0.05 vs the corresponding control.
