Fig. 4: RBM24 promotes Runx1t1 expression by stabilizing its mRNA.

a J82 cells were transfected with the RBM24 overexpression vector pWP-RBM24, or 253 J cells were transfected with shRBM24. The expression of Runx1t1 mRNA was measured by RT-qPCR. *P < 0.05 vs. the corresponding control. b Cells were treated as in (a) and then exposed to actinomycin D for 0, 2, 4, and 8 h. The mRNA expression of Runx1t1 was detected by using qRT-PCR. *P < 0.05 vs. the corresponding control. c 253 J cells were transfected with pWPI-RBM24 or empty vector. Western blotting was used to measure the precipitation efficiency of the RBM24 antibody. d RNA-binding protein immunoprecipitation (RIP) PCR was used to test the interaction between the RBM24 protein and Runx1t1 mRNA. e Different probes were used for oligo-pulldown, and western blot analysis was used to detect RBM24 in the pulldown precipitate. f 253 J cells were transfected with shRunx1t1, or J82 cells were transfected with pWP-Runx1t1. Western blot analysis was used to detect the protein levels of Runx1t1, RBM24 and CDK4. g Cells were prepared as in (f), and RT-qPCR was used to detect RBM24 mRNA expression.