Fig. 5: miR-19b promotes fracture healing in mice through the KLF5/β-catenin axis.
Mice in the femoral fracture model were injected with transduction reagents after the operation. a On the 7th day after injection and in week 0 after fracture, calli were harvested from mice in each group, and the level of miR-19b was measured by PCR to confirm the transduction efficiency of the miR-19b agomir in mice. *p < 0.05 versus the control group. b In week 0 after fracture, calli were harvested from mice in each group, and the protein levels of WWP1, Smurf2, KLF5, and β-catenin were measured by Western blot analysis, with β-actin as the internal reference protein. *p < 0.05 versus the control group, #p < 0.05 versus the miR-19b agomir group, NS > 0.05. c Representative X-ray images acquired to monitor femoral healing and changes over time at weeks 0 and 4 after fracture. d At the 4th week after femoral fracture, callus sections were stained with HE/Alcian blue, and the histology of the fracture calli was observed. Blue indicates cartilage, and pink indicates mineralized callus area. The experimental results are measurement data and are expressed as the mean ± standard deviation values. For multiple comparisons, one-way ANOVA followed by Tukey’s post hoc test was performed. n = 5 in the weeks 0 and 4 after fracture.
