Fig. 5: ERα expression attenuates the translocation of YAP via phosphorylation of MST1/2. | Experimental & Molecular Medicine

Fig. 5: ERα expression attenuates the translocation of YAP via phosphorylation of MST1/2.

From: YAP inactivation in estrogen receptor alpha-positive hepatocellular carcinoma with less aggressive behavior

Fig. 5

a Expression of YAP and phosphorylation of YAP at Ser109 and Ser127 were shown by Western blot analysis. b GSEA results showing the enriched expression of Hippo pathway-related genes. NES normalized enrichment score. c Levels of phospho-MST1/2 (T183/180), MST1, phospho-LATS1 (S1079), and LATS1 were shown by Western blot analysis. d ESR1 siRNA (50 nM) was used to treat ERα (+) HCC cell lines, and the phosphorylation of YAP (Ser127 and S109) and MST1/2 (T183/180) was shown by Western blot analysis. e Bar plots showing the normalized RLU values of the 8xGTIIC construct in the absence of ERα resulting from transfection of ESR1 siRNA (50 nM) in HepG2 (left) and Hep3B (right) liver cancer cells. The pGL3.0-basic vector (Ctrl) and NC (nontarget control) siRNA were used as controls. Statistical significance is indicated (*p < 0.05, **p < 0.01, and ***p < 0.001, Student’s t-test). f Immunohistochemical analysis of YAP showing its nuclear and cytoplasmic expression in ERα (−) and ERα (+) HCCs (left). Boxplots showing significant differences in nuclear and cytoplasmic expression of the YAP protein between ERα (−) and ERα (+) HCCs (p < 0.05, Fisher’s exact test; right).

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