Fig. 3: Silencing KDM4A increases the H3K9me3 level to enhance HIF1α methylation to inhibit NPC cell proliferation, migration, and invasion and induce apoptosis. | Experimental & Molecular Medicine

Fig. 3: Silencing KDM4A increases the H3K9me3 level to enhance HIF1α methylation to inhibit NPC cell proliferation, migration, and invasion and induce apoptosis.

From: Histone demethylase KDM4A plays an oncogenic role in nasopharyngeal carcinoma by promoting cell migration and invasion

Fig. 3

a, RT-qPCR detection of KDM4A and HIF1α mRNA expression in each group of cells. b, Western blot analysis of KDM4A, H3K9me3, and HIF1α protein expression in each group of cells. c, ChIP detection of the enrichment of H3K9me3 in the HIF1α promoter region in each group of cells. d, MTT assay detection of cell proliferation in each group. e, Transwell assay detection of the cell migration ability of each group. f, Transwell assay for cell invasion ability. g, Flow cytometry detection of apoptosis in each group. h, Western blot analysis of the expression of cell proliferation (Ki67, cyclin D1), migration (MMP-2, MMP-9), and apoptosis (Bcl-2, Bax)-related proteins in each group. *p < 0.05 vs. cells treated with si-NC + OE-NC, # p < 0.05 vs. cells treated with si-NC + OE-HIF1α. NS meant no significant difference. The measurement data are expressed as the mean ± standard deviation. One-way ANOVA was used for multigroup comparisons, and cell viability at different time points was compared by two-way ANOVA. The experiment was repeated three times independently.

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