Fig. 5: DDIT4 activates the mTOR signaling pathway to promote the proliferation, migration, and invasion and inhibit the apoptosis of NPC cells. | Experimental & Molecular Medicine

Fig. 5: DDIT4 activates the mTOR signaling pathway to promote the proliferation, migration, and invasion and inhibit the apoptosis of NPC cells.

From: Histone demethylase KDM4A plays an oncogenic role in nasopharyngeal carcinoma by promoting cell migration and invasion

Fig. 5

a Western blot analysis of mTOR, phosphorylated mTOR, 4EBP1, and phosphorylated 4EBP1 protein expression in clinical NPC tissues and adjacent tissues. b RT-qPCR detection of DDIT4 mRNA expression in each group of cells. c Western blot analysis of the protein expression of DDIT4, mTOR, phosphorylated mTOR, 4EBP1, and phosphorylated 4EBP1 in each group of cells. d Detection of cell proliferation in each group by MTT assay. e Detection of cell migration ability in each group by Transwell assay. f Detection of the cell invasion ability of each group by Transwell assay. g Detection of apoptosis in each group by flow cytometry. h Analysis of the protein expression of cell proliferation (Ki67, cyclin D1), migration (MMP-2, MMP-9), and apoptosis (Bcl-2, Bax)-related factors in each group by Western blot analysis. *p < 0.05 vs. control cells or cells cotreated with OE-D-NC and PBS, # p < 0.05 vs. cells cotreated with OE-D-NC and everolimus. NS meant no significant difference. The measurement data are expressed as the mean ± standard deviation. Data between cancer tissues and adjacent tissues were compared using paired t tests. One-way ANOVA was used for multigroup comparisons, and cell viability at different time points was compared using two-way ANOVA. For patients, n = 55. The experiment was repeated three times independently.

Back to article page