Fig. 2: The LXXLL motif of PLD2 interacts with the C-terminal region essential for SIRT1 activity. | Experimental & Molecular Medicine

Fig. 2: The LXXLL motif of PLD2 interacts with the C-terminal region essential for SIRT1 activity.

From: Phospholipase D2 is a positive regulator of sirtuin 1 and modulates p53-mediated apoptosis via sirtuin 1

Fig. 2

a Schematic representation of various deletion mutant SIRT1 constructs. HEK293 cells were transfected with PLD2 and SIRT1 deletion mutants, and the lysates were analyzed by immunoprecipitation and immunoblotting using the indicated antibodies. b Schematic representation of various GST-PLD2 fragment fusion constructs. GST pull-down assays using equal amounts of GST or GST-PLD2 fragment fusion proteins immobilized on glutathione sepharose 4B beads were performed with HCT116 cell lysates and immunoblotting was performed with antibodies against SIRT1. The amount of GST fusion proteins was visualized by Coomassie brilliant blue (CBB) staining. c Schematic representation of LXXAA mutants of PLD2 and PX domain-deleted PLD2 (ΔPX). HEK293 cells were cotransfected with SIRT1 and the indicated PLD2 constructs and analyzed by immunoprecipitation and immunoblotting. The results are representative of at least three independent experiments.

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